Access of cellulase to cellulose and lignin for poplar solids produced by leading pretreatment technologies.
Identifieur interne : 003405 ( Main/Exploration ); précédent : 003404; suivant : 003406Access of cellulase to cellulose and lignin for poplar solids produced by leading pretreatment technologies.
Auteurs : Rajeev Kumar [États-Unis] ; Charles E. WymanSource :
- Biotechnology progress [ 1520-6033 ]
Descripteurs français
- KwdFr :
- MESH :
- composition chimique : Cellulase, Cellulose, Lignine, Populus, Protéines végétales.
- enzymologie : Populus.
- méthodes : Biotechnologie.
- Adsorption.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Cellulase, Cellulose, Lignin, Plant Proteins.
- chemistry : Populus.
- enzymology : Populus.
- methods : Biotechnology.
- Adsorption.
Abstract
Adsorption of cellulase on solids resulting from pretreatment of poplar wood by ammonia fiber expansion (AFEX), ammonia recycled percolation (ARP), controlled pH, dilute acid (DA), flowthrough (FT), lime, and sulfur dioxide (SO(2)) and pure Avicel glucan was measured at 4 degrees C, as were adsorption and desorption of cellulase and adsorption of beta-glucosidase for lignin left after enzymatic digestion of the solids from these pretreatments. From this, Langmuir adsorption parameters, cellulose accessibility to cellulase, and the effectiveness of cellulase adsorbed on poplar solids were estimated, and the effect of delignification on cellulase effectiveness was determined. Furthermore, Avicel hydrolysis inhibition by enzymatic and acid lignin of poplar solids was studied. Flowthrough pretreated solids showed the highest maximum cellulase adsorption capacity (sigma(solids) = 195 mg/g solid) followed by dilute acid (sigma(solids) = 170.0 mg/g solid) and lime pretreated solids (sigma(solids) = 150.8 mg/g solid), whereas controlled pH pretreated solids had the lowest (sigma(solids) = 56 mg/g solid). Lime pretreated solids also had the highest cellulose accessibility (sigma(cellulose) = 241 mg/g cellulose) followed by FT and DA. AFEX lignin had the lowest cellulase adsorption capacity (sigma(lignin) = 57 mg/g lignin) followed by dilute acid lignin (sigma(lignin) = 74 mg/g lignin). AFEX lignin also had the lowest beta-glucosidase capacity (sigma(lignin) = 66.6 mg/g lignin), while lignin from SO(2) (sigma(lignin) = 320 mg/g lignin) followed by dilute acid had the highest (301 mg/g lignin). Furthermore, SO(2) followed by dilute acid pretreated solids gave the highest cellulase effectiveness, but delignification enhanced cellulase effectiveness more for high pH than low pH pretreatments, suggesting that lignin impedes access of enzymes to xylan more than to glucan, which in turn affects glucan accessibility. In addition, lignin from enzymatic digestion of AFEX and dilute acid pretreated solids inhibited Avicel hydrolysis less than ARP and flowthrough lignin, whereas acid lignin from unpretreated poplar inhibited enzymes the most. Irreversible binding of cellulase to lignin varied with pretreatment type and desorption method.
DOI: 10.1002/btpr.153
PubMed: 19504581
Affiliations:
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From this, Langmuir adsorption parameters, cellulose accessibility to cellulase, and the effectiveness of cellulase adsorbed on poplar solids were estimated, and the effect of delignification on cellulase effectiveness was determined. Furthermore, Avicel hydrolysis inhibition by enzymatic and acid lignin of poplar solids was studied. Flowthrough pretreated solids showed the highest maximum cellulase adsorption capacity (sigma(solids) = 195 mg/g solid) followed by dilute acid (sigma(solids) = 170.0 mg/g solid) and lime pretreated solids (sigma(solids) = 150.8 mg/g solid), whereas controlled pH pretreated solids had the lowest (sigma(solids) = 56 mg/g solid). Lime pretreated solids also had the highest cellulose accessibility (sigma(cellulose) = 241 mg/g cellulose) followed by FT and DA. AFEX lignin had the lowest cellulase adsorption capacity (sigma(lignin) = 57 mg/g lignin) followed by dilute acid lignin (sigma(lignin) = 74 mg/g lignin). AFEX lignin also had the lowest beta-glucosidase capacity (sigma(lignin) = 66.6 mg/g lignin), while lignin from SO(2) (sigma(lignin) = 320 mg/g lignin) followed by dilute acid had the highest (301 mg/g lignin). Furthermore, SO(2) followed by dilute acid pretreated solids gave the highest cellulase effectiveness, but delignification enhanced cellulase effectiveness more for high pH than low pH pretreatments, suggesting that lignin impedes access of enzymes to xylan more than to glucan, which in turn affects glucan accessibility. In addition, lignin from enzymatic digestion of AFEX and dilute acid pretreated solids inhibited Avicel hydrolysis less than ARP and flowthrough lignin, whereas acid lignin from unpretreated poplar inhibited enzymes the most. Irreversible binding of cellulase to lignin varied with pretreatment type and desorption method.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19504581</PMID><DateCompleted><Year>2009</Year><Month>09</Month><Day>14</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1520-6033</ISSN><JournalIssue CitedMedium="Internet"><Volume>25</Volume><Issue>3</Issue><PubDate><MedlineDate>2009 May-Jun</MedlineDate></PubDate></JournalIssue><Title>Biotechnology progress</Title><ISOAbbreviation>Biotechnol Prog</ISOAbbreviation></Journal><ArticleTitle>Access of cellulase to cellulose and lignin for poplar solids produced by leading pretreatment technologies.</ArticleTitle><Pagination><MedlinePgn>807-19</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/btpr.153</ELocationID><Abstract><AbstractText>Adsorption of cellulase on solids resulting from pretreatment of poplar wood by ammonia fiber expansion (AFEX), ammonia recycled percolation (ARP), controlled pH, dilute acid (DA), flowthrough (FT), lime, and sulfur dioxide (SO(2)) and pure Avicel glucan was measured at 4 degrees C, as were adsorption and desorption of cellulase and adsorption of beta-glucosidase for lignin left after enzymatic digestion of the solids from these pretreatments. From this, Langmuir adsorption parameters, cellulose accessibility to cellulase, and the effectiveness of cellulase adsorbed on poplar solids were estimated, and the effect of delignification on cellulase effectiveness was determined. Furthermore, Avicel hydrolysis inhibition by enzymatic and acid lignin of poplar solids was studied. Flowthrough pretreated solids showed the highest maximum cellulase adsorption capacity (sigma(solids) = 195 mg/g solid) followed by dilute acid (sigma(solids) = 170.0 mg/g solid) and lime pretreated solids (sigma(solids) = 150.8 mg/g solid), whereas controlled pH pretreated solids had the lowest (sigma(solids) = 56 mg/g solid). Lime pretreated solids also had the highest cellulose accessibility (sigma(cellulose) = 241 mg/g cellulose) followed by FT and DA. AFEX lignin had the lowest cellulase adsorption capacity (sigma(lignin) = 57 mg/g lignin) followed by dilute acid lignin (sigma(lignin) = 74 mg/g lignin). AFEX lignin also had the lowest beta-glucosidase capacity (sigma(lignin) = 66.6 mg/g lignin), while lignin from SO(2) (sigma(lignin) = 320 mg/g lignin) followed by dilute acid had the highest (301 mg/g lignin). Furthermore, SO(2) followed by dilute acid pretreated solids gave the highest cellulase effectiveness, but delignification enhanced cellulase effectiveness more for high pH than low pH pretreatments, suggesting that lignin impedes access of enzymes to xylan more than to glucan, which in turn affects glucan accessibility. In addition, lignin from enzymatic digestion of AFEX and dilute acid pretreated solids inhibited Avicel hydrolysis less than ARP and flowthrough lignin, whereas acid lignin from unpretreated poplar inhibited enzymes the most. Irreversible binding of cellulase to lignin varied with pretreatment type and desorption method.</AbstractText><CopyrightInformation>2009 American Institute of Chemical Engineers</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kumar</LastName><ForeName>Rajeev</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Thayer School of Engineering, Dartmouth College, NH 03755, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wyman</LastName><ForeName>Charles E</ForeName><Initials>CE</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D023362">Evaluation Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biotechnol Prog</MedlineTA><NlmUniqueID>8506292</NlmUniqueID><ISSNLinking>1520-6033</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>9004-34-6</RegistryNumber><NameOfSubstance UI="D002482">Cellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.4</RegistryNumber><NameOfSubstance UI="D002480">Cellulase</NameOfSubstance></Chemical><Chemical><RegistryNumber>OP1R32D61U</RegistryNumber><NameOfSubstance UI="C109691">microcrystalline cellulose</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000327" MajorTopicYN="N">Adsorption</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001709" MajorTopicYN="N">Biotechnology</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002480" MajorTopicYN="N">Cellulase</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002482" MajorTopicYN="N">Cellulose</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>6</Month><Day>9</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>6</Month><Day>9</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>9</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19504581</ArticleId><ArticleId IdType="doi">10.1002/btpr.153</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>New Hampshire</li></region></list><tree><noCountry><name sortKey="Wyman, Charles E" sort="Wyman, Charles E" uniqKey="Wyman C" first="Charles E" last="Wyman">Charles E. Wyman</name></noCountry><country name="États-Unis"><region name="New Hampshire"><name sortKey="Kumar, Rajeev" sort="Kumar, Rajeev" uniqKey="Kumar R" first="Rajeev" last="Kumar">Rajeev Kumar</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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